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The Mesoscale K15564U-4 is a 4-channel 16-bit 250 kS/s simultaneous sampling data acquisition system It has 4 analog input channels with software-selectable input ranges of 10 V 5 V 2 V and 1 V The system can stream acquired data to PC memory at rates up to 250 kS/s per channel It connects to the host computer via USB 2 0 interface and is powered via this USB connection The K15564U-4 is compatible with Windows operating systems Key features include 16-bit A/D conversion software-selectable input ranges simultaneous sampling on all channels and streaming acquired data to PC memory The compact device is bus-powered for portability
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A DNA sequence encoding the Influenza A virus (A/California/04/2009 (H1N1)) Neuraminidase / NA (ACP41107.1) (Ile30-Lys469) was expressed with a vasodilator-stimulated phosphoprotein tetramerization domain at the C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) also known as 2019-nCoV is a positive-sense single-stranded RNA virus It caused coronavirus disease 2019 (COVID-19) Nucleocapsid Protein is the most abundant structural protein of the coronavirus which is associated with the nucleic acid The sublineage B 1 617 2 has been redesignated as a variant of concern (VOC-21APR-02) in May 2021 which spreads more quickly than the original version of the virus
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SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases Recently the new B 1 1 529 variant was confirmed in South Africa and preliminary evidence suggests an increased risk of reinfection with this variant The B 1 1 529 variant was first reported to WHO on 24 November 2021 and WHO has designated this variant as a VOC (Variant of Concern) named Omicron There are more than 30 mutations in the spike protein
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A σ receptor ligand with anticancer and antiviral activites binds to σ1 and σ2 receptors (Kis 13 and 0.28 nM respectively in MCF-7 cell membranes) selective for σ1 and σ2 receptors over hERG channels (Ki 1000 nM) inhibits growth of MCF-7 and multidrug-resistant MCF-7/adr cells (IC50s 25 and 15 nM respectively) decreases basal P-gp levels in the same cells reduces SARS-CoV-2 infectivity in Vero E6 cells (IC90 278 nM)
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The NEBNext ARTIC kits were developed in response to the critical need for reliable and accurate methods for sequencing SARS-CoV-2. These kits, for long and short read sequencing, were based on the original work of the ARTIC Network. The ARTIC SARS-CoV-2 sequencing workflow is a multiplexed amplicon-based whole-viral-genome sequencing approach. NEBNext ARTIC kits include primers and reagents for RT-PCR from SARS-CoV-2 gRNA and downstream library preparation. The balanced primers and reagents for RT-PCR deliver improved uniformity of amplicon yields from gRNA across a wide copy number range. The NEBNext ARTIC SARS-CoV-2 Companion Library Prep Kit (Oxford Nanopore Technologies) is compatible with a broad range of total RNA input amounts and is used alongside kits from Oxford Nanopore Technologies to generate targeted, high quality libraries for next-generation sequencing on the Oxford Nanopore platform.
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The Meso Scale Discovery V-PLEX SARS-CoV-2 Panel 1 Mouse IgG Kit is an electrochemiluminescence immunoassay that detects IgG antibodies to SARS-CoV-2 in mouse serum and plasma samples This assay uses the S1 and receptor binding domain (RBD) antigens to detect antibodies that bind to the spike protein The kit includes a 96-well plate coated with antigens detection antibody read buffer wash buffer calibrators and controls Results are obtained in 3 5 hours using an MSD instrument This assay allows high-throughput sensitive and quantitative measurement of SARS-CoV-2 IgG antibodies
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The V-PLEX Human IL-6 Kit is a multiplex immunoassay kit from Meso Scale Diagnostics that allows for the quantitative measurement of human interleukin 6 (IL-6) in serum plasma and cell culture supernatants The assay utilizes electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels calibrators diluents wash buffers read buffer and plate seals The assay has a typical sensitivity of 0 10 pg/mL and an upper limit of quantification of 1500 pg/mL for serum/plasma samples
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